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It is therefore likely that the intracellular trehalose accumulation may be important for multiple stress tolerance in our strains.
Further studies are, however, required to clarify the precise molecular mechanisms underlying multiple stress tolerance of these strains.
These results suggest that the continuous expression of HSP genes may be one of those molecular mechanisms important for multiple stress tolerance in the C3751 strain.
The main objectives of this study were to screen for yeast strains displaying tolerance to multiple stresses present during high-temperature ethanol fermentation, i.e., heat, ethanol, osmotic, oxidative, and cell wall stresses, and to investigate cellular mechanisms responsible for multiple stress tolerance in these strains.
Our experiments further suggest ROS scavenging to be the possible mechanism for multiple stress tolerance conferred by OsMT1e-P.
In addition, overexpression of ABF2, ABF3 has been shown to affect multiple stress tolerance, implying that PtrABF might function in the same way as ABFs of Arabidopsis.
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We conclude that EsDREB2B is a promising candidate gene for the development of crops with multiple stress tolerances.
Among the 2,611 differently expressed genes in these two strains, many were involved in the trehalose metabolism pathways, antioxidative factors, and membrane composition biosynthesis that are closely related to multiple stress-tolerance and fermentation characteristics.
Multiple-stress tolerance of the type strain Geodermatophilus obscurus was already described by Gtari et al. [ 11], suggesting a correlation between tolerance profiles to desiccation, mitomycin C, hydrogen peroxide, and ionizing- and UV-radiation.
ZAT12 encodes a C2H2 zinc finger protein which confers multiple abiotic stress tolerance to plants.
Root architecture is one trait among others for breeders, and it will be necessary to concurrently manipulate or introgress in addition to genes related to root architecture, those for disease resistance, multiple abiotic stress tolerance, grain quality, etc.
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