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A multiple stress combination approach could be necessary to decipher all of the pathways involved in HD stress response.
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Therefore in an attempt to mimic the conditions that the pathogen might encounter in the host, we applied a combination of these four stresses (multiple stress) on Mtb in culture.
Currently, a unique climate chamber to simulate multiple stress factors on terrestrial organisms is constructed at BOKU University and will allow reproducing different environmental conditions separately or in combination with each other.
To test if the signature concept could be applied to multiple stress treatment conditions, we conducted an expression profiling experiment on plants exposed to heat, drought, and then heat and drought in combination (refer to Materials and Methods).
She had multiple stress fractures, a knee injury and shin splints.
Twenty five patients underwent radionuclide ventriculography during a multiple stress challenge.
Multiple stress systems regulate action selection.
This combination of multiple stresses can have a great adverse impact on plants (Zhang et al. 2010); high temperature strongly affects water retentions when water is limiting (Machado and Paulsen 2001).
We found that when Mtb was subjected to the combination of multiple stresses in vitro, it developed higher degree of phenotypic resistance to Rif and INH indicating that a higher percentage of the mycobacterial population entered a dormant state.
Moreover, whereas the identity of the cell death trigger(s) remains to be determined, it indicates that a combination of multiple stresses in cells that do not respond to TMZ allows a switch to activate cell death pathways.
Without this type of information it is difficult to determine whether the observed transcriptional response reflects a new stress-signaling pathway, a physiologic level of activity of a known stress-signaling pathway, or a combination of multiple stress-signaling pathways activated in response to the unfolded DD.
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