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The rates we determined in this study should be viewed as the minimum incidence because multiple stool sampling is not a standard practice in our region, and many patients may have had these diseases without having a stool sample submitted for parasitological testing.
Multiple stool sampling, ideally over several consecutive days, increases the sensitivity [ 136], as well as the use of different concentration techniques, which are based on sedimentation (e.g. formalin-ether concentration technique), flotation or a combination of both (e.g. McMaster technique and FLOTAC) [ 135, 137- 139].
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Collecting multiple stool samples from asymptomatic persons may also be a challenge.
While testing for detection of protozoan parasites from HIV cases, it needs to collect multiple stool samples if feasible.
Sensitivity is increased by preparing a faecal thick smear using the Kato-Katz (KK) method (Gryseels 2006) or by examining multiple stool samples (Feldmeier 1993).
To estimate the effect of the quality of the reference standard, we accepted studies using microscopy on a single stool sample (lower-quality reference standard) and studies performing microscopy on multiple stool samples (higher-quality reference standard).
Your doctor may ask you for multiple stool samples to be positive of a diagnosis.
In conclusion, fast and reliable diagnosis of Blastocystis infection in routine clinical parasitology is possible by advanced microscopy on multiple fixed stool samples with immediate fixation after production of stools.
It is well known that the laboratory diagnosis of S. stercoralis requires multiple fresh stool samples using concentration techniques and/or copro-culture in order to improve detection rates [ 32].
Reference standards that used multiple urine or stool samples or multiple slides or smears were classified as higher-quality reference standards.
Yes: if measures to increase sensitivity are used (eg concentration techniques, multiple slides examined, stool sampled over a number of days. The recommended reference std for microscopy is one carried out on 3 stools or 3 urine samples (grading as follows: 1 sample; poor; 2 samples; moderate; 3 samples; good).
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