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The DNA sequence that is amplified is determined by the design of oligonucleotide primers, short pieces of synthesised DNA that bind to either end of the sequence and form the starting point for DNA replication by DNA polymerase.
A primer is a short DNA sequence that serves as a starting point for DNA synthesis.
During HRCA, multiple nicking events create new starting points for replication.
DNA of the three grapevine downy mildew isolates was extracted from samples of the infected leaf that served as a starting point for subsequent replication.
The starting points for the unwinding process are not only DSBs induced by reactive oxygen species (ROS), irradiation, or chemical compounds, but also replication forks or chromosome ends.
The starting point for the SoVI® replication was the Brazilian state of Paraná, one of the three states in the South region.
Therefore, our analysis provides a key starting point for understanding the subsequent evolutionary history of the eukaryotic DNA replication machinery.
This work also provided a starting point for the enzymatic synthesis of DNA (an alternating polymer of sugar and phosphate).
We consider the existing version of CellNOptR as a robust and flexible starting point for multiple developments.
Mückstein et al. [ 15] suggest the use of the method as a starting point for improved multiple sequence alignment procedures.
The chromosomes replicate from multiple starting-points (origins of replication) using DNA polymerases that resemble the equivalent eukaryotic enzymes.
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