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Using other multiple staining protocols (i.e., using immunofluorescence techniques), the image information provided could be enhanced, thus providing more input to advanced image processing techniques.
Indeed, with the advantage of tissue from genetically engineered XIAP-deficient mice, we have observed significant staining, and therefore a lack of specificity, with a number of commercially available antibodies and with multiple staining protocols (unpublished observations).
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Overall, dextran conjugates provide high-definition labeling of an extensive vagal motor pool in the GI tract, and offer considerable versatility when multiple-staining protocols are needed to elucidate the complexities of the innervation of the gut.
Thus, a system for multiple staining using stable fluorescent dyes is crucial to develop a new diagnostic protocol for the pathological examination of cancer.
Using a computer-interfaced microscope stage, image analysis software and a 3-CCD colour video camera, multiple images are digitized, combined to form a photo-montage and revisited after each of the three staining protocols.
developed tissue preparation and staining protocols.
Consultation is available on selection of appropriate staining protocols for research projects.
C.C.O.M. established staining protocols for three-dimensional structured illumination microscopy (3D SIM).
Optimal for some immunohistochemical staining protocols, preservation of fluorescent cell markers (GFP, tomato) and lipids in tissues.
In this article, the reagents and staining protocols for immunophenotyping are discussed.
Several staining protocols were examined to yield optimized immunolabeling.
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