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This work probed the spatial distribution of extracellular polymeric substances, cells in the original wastewater flocs, and the yielded fragments following weak ultrasonication using a process of multiple staining and a confocal laser scanning microscope technique.
Because the MCC is designed to allow collection and analysis of non-adherent cell populations, multiple staining steps can be applied in parallel to a given CTC population without losing any of the population.
This process was carried out on multiple egg chambers (n≥10) and repeated in multiple staining experiments.
Multiple staining experiments are represented as color density plots [63] with linear scales used for both forward (FSC) and side scatter (SSC) and logarithmic scales used for fluorescence parameters (Log density: 50%).
Hopefully, this multiple staining method will improve the accuracy of the diagnosis of various diseases.
However, multiple staining would be technically challenging in handling the archived paraffin-embedded tissues.
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This allows multiple stains to be used in combination within a single sample.
The color deconvolution algorithm detects and separates multiple stains by analyzing their absorption spectra and relative contributions to areas containing two or more overlapping stains.
Image analysis and statistical calculations were performed using the Progenesis SameSpots software (NonLinear Dynamics, Newcastle, UK) and the 'Multiple stains per gel without internal standards' comparison method.
Recent validation studies for primary diagnosis use the "broader scope" approach [ 52– 54], where diagnoses on multiple tissue types from multiple organs and using multiple stains are compared between optical and digital microscopy.
In order to investigate the differentiation capacity of hMSC after transplantation into the infarcted heart multiple antibodies staining was performed.
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