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Multiple stable clones were used to rule out potential clonal effects.
Multiple stable clones were used to eliminate potential clonal effects.
Transfected cells were put through two rounds of selection using appropriate antibiotic (puromycin 2 μg/ml, G418 400 μg/ml) to select multiple stable clones.
Typical traces are shown for a parental CALU-3 cell monolayer and for multiple stable clones expressing ΔF-CFTR as an engineered heterozygous cell model co-expressing WT-CFTR and ΔF-CFTR endogenously.
Similar(56)
To investigate whether Tcl1 is a major mediator of the effects of Oct3/4 on the proliferation of ES cells, we established multiple stable cell clones (Tcl1- 2A1, Tcl1- 2D5, and Tcl1- 1A6) by transfecting a plasmid vector constitutively expressing Tcl1 into ZHBTc4 ES cells.
#1 and #2 are independent stable clones.
Stable clones were selected using 300 µg ml−1 G418 (Sigma Aldrich).
All cells and stable clones were routinely tested for mycoplasma and found to be negative.
Hence, the "multiple stable steady states" in21 are actually metastable.
Two stable clones of EPC transfected with pcDNA3-LUC and four clones transfected with pcDNA3-LP-LUC were isolated.
For generating a CBX6-expressing stable cell line, 1 mg/mL G418 (Gibco) was added to the culture medium to screen for stable clones.
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