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"Some [collectors] buy multiple spots to increase their odds of landing something good.
Fourkas adds that further improvements should speed up the process by lowering the amount of power required to write one spot and allowing multiple spots to be written simultaneously.
Fluorescence microscopy studies in H. polymorpha revealed that GFP-tagged Dnm1 is not evenly distributed in the cell, but rather present in multiple spots to which Mdv1 colocalizes.
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Both proteins were present in multiple spots, up to five in the case of CAP0037 and at least up to four in the case of CAP0036 (Fig. 2, Table 1).
Data from multiple spots mapping to the same UNIGENE cluster ID were averaged across the multiple spots.
Unique proteins, when analyzed on the 2D gel, often show multiple spots due to posttranslational modifications.
Multiple spots corresponding to phosphorylated and unphosphorylated MLC-2 isoforms were observed by 2DE.
In addition, in a study using LIVE/DEAD BacLight, multiple spots considered to represent microcolonies became conspicuous at half the VCM concentration that caused color change, and close examination of each spot confirmed that dead bacteria were surrounded by live ones.
Raman spectra from multiple spots were collected to perform the statistical analysis of the samples.
Note: some proteins showed in the 2-DE as multiple spots, probably due to their isoforms and/or modifications.
Individual proteins were reflected by multiple spots most likely due to posttranslational modification leading to shifts in the 2-DE.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com