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In case of multiple spots for the same gene, only the most significant (highest F-statistic), was selected for further analysis.
The multiple spots for the same protein are most likely due to post-translational modifications (glycosylation, phosphorylation) that cause shift in protein mobility.
If you need to choreograph multiple spots for cutting in, a longer song may better suit your needs.
Try to find a spot that is suitable to your taste where you can relax by yourself or with friends, or even get multiple spots for multiple reasons!
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Multiple spots were made for each strain and the whole population of one spot was determined every 24 hr for 144 hr (the aggregate population of ∼100 colonies growing above a tester lawn) by excising an entire spot and suspending cells in 1.0 ml of NCE medium.
Such activity could lead to post-translational modification in phloem and might account for the multiple spots on gels that were found for some of the identified proteins.
The peak value of this distribution is recorded as the 'label' for that particular spot and the process is repeated for multiple spots within the image.
The measurements for multiple spots can be added to the query and saved as a data file in text format for further statistical analysis.
Proteins can migrate to multiple spots on 2D gels for a variety of reasons, including differential protein processing and posttranslational or artefactual modifications.
The 12 housekeeping genes with multiple spots (ten per array) were used for the determination of intra-array variation.
Because full sections were evaluated for each specimen, multiple spots with diameters of 0.6 mm were analyzed to obtain a representative level of protein expression.
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