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However, you can presumably set up shop at multiple spots and keep raising money ad infinitum.
Five of the proteins identified by MS were found in multiple spots and one protein showed differential expression of two of its subunits (fibrinogen beta and gamma chains).
In control cells all the metaphases have spots of DSas-4 protein at the poles (96% with 2 spots, 4% with multiple spots,) and as expected, none of the DSas-4−/− cells presented spots of DSas-4 protein (n = 50 for each line).
Approximately 40% of the identified proteins were detected in multiple spots and had different isoelectric points (pIs) and molecular weights (MWs), suggesting the presence of isoforms and post-translational modifications or that these proteins were translated from different products of paralogous genes within a multigene family (Table 1) [ 19].
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We preferred combination of multiple spotting and dye-swap normalization, which ensured robust normalization and accurate detection of differential expression at low ratios.
XRF spectra taken from multiple spots inside and outside the area of dress do not demonstrate any difference in the elemental composition suggesting carbon black.
However, even for individual genes, a given array used multiple probes and multiple spots (see "Materials and Methods"), so the changes cannot be "chance".
For novel spots, post-translational modification is likely; previously, we identified pyruvate phosphate dikinase to be phosphorylated after UV-B of WPI [ 4], and this protein was also identified in multiple spots in irradiated and shielded leaf samples.
According to our experience, Stouffer's method is the only available method yielding plausible results if genes are resampled by multiple spots on a chip and if these might exhibit inconsistent fold-changes and significantly varying expression levels.
STIR and T2 series show multiple spots of high signal intensity and series after contrast show enhancement.
Whole-body MRI STIR can be used for screening Metastasis Multifocal lesions, no inflammation parameters CRMO STIR and T2 series show multiple spots of high signal intensity, and series after contrast show enhancement.
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