Consequently, the assembly produces two sets of sequences: a theoretically non-redundant set of contigs, and a set of isotigs which are candidate long- or full-length transcripts from multiple splice variants of genes, and contains some partially redundant sequence segments.
Moreover, multiple splice isoforms of PLA2G4C exist, differing in transcript length, presence of certain exons and overlapping exons with different boundaries (AceView, NCBI, http://www.ncbi.nlm.nih.gov/IEB/Research/Acembly/).nih.gov/IEB/Research/Acembly/
The features of the explicit alternative splicing prediction methods: k-mer counts, exon lengths, and sequence conservation are used to predict multiple splice sites and intron retention events along with cassette exons and constitutive exons.
Many species across the vertebrate lineage contain multiple splice variants of each gene type, which are characterized by length and amino termini.
When multiple splice vari-ants of FGF5 and Hr were available, only the full-length form from a species was used in subsequent analyses.
It is expected, however, that intronic full-length (FL) L1 insertions have a much higher exonization potential since they contain multiple splice sites and, for example, only FL L1s will include 5' promoter regions containing splice sites.
The splice lengths were relatively large to test realistic splice lengths used in the field (longer than 30db in most tests).
Alternative splicing analysis of Col2a1 splice forms has often relied upon semi-quantitative PCR, using a single set of PCR primers to amplify multiple splice forms.
It was demonstrated that large flexural capacity can be achieved with relatively short lap splice lengths.
This investigation showed that hCE expressed multiple splice variants of PMCA isoforms.
