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The sequences from the adult brain, spinal cord, and E17.5 embryonic tissues were unreadable, presumably due to the presence of multiple splice isoforms.
Both proteins are expressed in multiple splice isoforms – alternative splicing occurs at their N-terminal regions and tissue- and development-specific isoforms have been described in human, rat and mouse (for brief discussion and original references see [21], [22], [23], [3].
Many of these proteins have multiple splice isoforms for which UniProt accession numbers are also listed.
For genes with multiple splice isoforms, the transcripts with the longest sequences were used for analysis.
Therefore the observed complex pattern of multiple splice isoforms can slightly vary between biological replicates.
CASC4, PPFIBP1, and SDCCAG8 are examples of ORFs that encode multiple splice isoforms that behave differently across the cell cycle.
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It exhibits multiple splicing isoforms and modulates the function of the haematopoietic cells.
Alternative splicing (AS) generates multiple splicing isoforms with different combination of exons thus dramatically increasing the molecular complexity of the expressed proteins [84].
In addition, multiple splicing isoforms of many genes may be present in the transcriptomes.
Taken together, these results suggest that Newbler v2.3 has a low rate of false positives in the prediction of multiple splicing isoforms.
HDR induced precise mutagenesis is particularly useful for generating null mutations of microRNAs and other non-coding RNAs, and for those genes with multiple splicing isoforms.
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