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Alternative splicing analysis of Col2a1 splice forms has often relied upon semi-quantitative PCR, using a single set of PCR primers to amplify multiple splice forms.
The DYRK1A mRNA has multiple splice forms.
OXR1 is a complex gene that is expressed as multiple splice forms.
CEACAM1 has multiple splice forms that are differentially expressed between epithelial and immune cells.
Both genes are large with multiple splice forms, and the two platforms did not target the same transcript sequences.
Genome-wide analyses of expressed sequences indicate that 40 60% of human genes have multiple splice forms [9].
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The protocadherin gene family has genomic structure similar to IGH and has multiple splicing forms and multiple alternative promoters.
We previously catalogued and evaluated expression of multiple splicing forms of TCF7L2 in several types of human tissue [21].
Finally, it is important to remember that the coexistence of nuclear receptors with multiple splicing forms could influence the formation of homo- and heterodimers, receptor posttranslational modifications, and localization at the membrane, which are all parameters that can likely contribute to the complex properties of BPA.
Each of the four PDE4 gene isoforms (PDE4 A-D) undergo extensive alternative splicing via alternative transcription initiation sites, producing unique amino termini and yielding multiple splice variant forms from each gene isoform termed long, short, super-short and truncated super-short.
The intracellular targeting and microdomain diversity of the PDE4 family suggests a functional expansion through the incorporation of different splice variant forms (long, short, super-short truncated super-short), as well as the alternative splicing of multiple splice variant 5' exons from each PDE4 gene isoform (observed in mammalian PDE4s).
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