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Furthermore, although in theory the activity of all cells filled with the Ca2+ indicator can be monitored, in practice some cells do not show clear fluorescence changes, even when multiple spikes are present.
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However, multiple spikes were observed in all neurons recorded at day 37.
In this case, trials with single and multiple spikes were equivalent.
Current clamp recordings showed changes in spike amplitude, frequency and duration and sequential firing of multiple spikes was found by day 37.
These results clearly show a steady ongoing process of electrical maturation for human in-vitro developing neurons, in which sequential firing of multiple spikes is achieved by day 37 following induction of IVND with the dual SMAD inhibition protocol.
The fine structure of the funnel at its virus-proximal site cannot be resolved in the tomograms given the ECT resolution, however, one possibility envisages a mouth opening ringed by fusion peptides from multiple spikes that are embedded in a micellar cap or similar non-bilayer structure.
The ARP of sequential spikes are measured by injecting multiple depolarization current pulses (3 ms and 5% above threshold levels) into the neurons following each of spikes.
Regardless of the approach chosen, for the sample problem reported herein, excellent resolution of multiple delta-like spikes is produced from MRE from noisy, diffuse data.
Male spikes are cylindrical.
Spikes spikes are super rare.
Spikes are helpful.
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