However, only a handful of examples of multiple soluble protein nanoarrays has been reported, and, to date, none of the techniques mentioned above have been used to nanopattern multiple purified membrane proteins (MPs).
Pancreatic stellate cells produce excessive amounts of ECM proteins (fibronectin, collagen I, III) as well as multiple soluble chemokines.
Elements thought to be unfavorable for the stability of gpBLT1 were extracted based on the stabilization elements established in soluble proteins, determined crystal structures of G-protein-coupled receptors (GPCRs), and multiple sequence alignment.
The electric charge of soluble proteins such as PGIs is a structural property brought by a large number of multiple amino acid residues and is involved in the adaptive evolution of several soluble proteins [e.g., [ 22- 24]], such as an acquisition of protein thermostability [ 25- 27].
Indeed, a large number of plastids carrying multiple transgene copies can exist in a cell, resulting in very strong chloroplast expression of up to 25% soluble proteins.
Moreover, a multiple construct approach applied to DDX3 has previously shown that constructs lacking the first 169 aa were perfectly expressed as soluble proteins [18].
The latter are able to bind and precipitate soluble proteins.
After removal of soluble proteins using sodium dodecyl sulfate, confocal microscopy was used to visualize the 3D distribution of independent ECM networks in multiple developing tissues, including the forelimb, eye, and spinal cord.
The supernatant contained soluble proteins.
For example, soluble proteins may aggregate and undergo autophagy as well as being degraded by the proteasome [19], and there may well be multiple enzymes capable of ubiquitinating any given protein.
