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Although the combination of multiple smear tests over three consecutive days resulted in the highest yield of smear microscopy (18 children), this approach required examining 1309 specimens and 237 hospitalization days.
DOI: http://dx.doi.org/10.7554/eLife.04177.033 In addition, chemical cross-linking of sDectin-1, followed by immunoblot analysis, generated multiple smear bands of similar pattern using B16F1 and 3LL cells, whereas bands were barely detectable with B16F10 cells or MEFs.
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Many patients had multiple smears, and these were processed independently, blind to the patient's identity.
In case multiple smears were taken during the study period, the most severe lesion was used for the analysis.
If women had only multiple normal smear results in the screened period, we analysed the time interval between the last smear before the diagnosis and at the time of diagnosis.
A recent study suggested that women with multiple inadequate smear test results may have a slightly increased risk of developing high-grade CIN over a period of 5 years in comparison to other women with adequate smear test results (Hock et al, 2003).
Mycobacteria were confirmed by multiple skin smears stained with Ziehl-Neelsen stain.
In case of multiple abnormal smears in the screening history (e.g., in case of repeat cytology after BMD), we accepted the first abnormal smear as the starting point.
Such studies should also investigate whether simpler modifications (e.g., parallel performance of multiple direct smears on a single sputum specimen) can similarly increase sensitivity and cost-effectiveness.
In conclusion, from our data the risk of pre-invasive cervical disease at the age of 50 years in women with a history of multiple negative smears between ages 40 and 49 years was moderately reduced.
As many as 20 of the 32 probes yielded several hundreds to thousands of positive double signals onto one BAC filter and multiple bands or smear onto the genomic southern blots indicating that the probes contained repetitive sequence elements.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com