Multiple unit sizes: Offering multiple size options allows for more flexibility in terms of items being stored.
Multiple sizes of paired end and mate pair libraries were sequenced to produce paired 100-bp reads with insert sizes ranging from 200 bp to 7 kb.
But, says Vucko, "multiple sizes are coming.
I tried on so many different swimsuits, in multiple styles, in multiple sizes.
Buy coils in multiple sizes.
This involves for instance, sequencing transcriptomes of multiple tissues and developmental stages at great depth and using different insert-size libraries.
Ribeiro et al. used multiple MP libraries with insert sizes ranging from 2 6 kb and were able to generate finished or near-finished assemblies for different bacterial genomes (Ribeiro et al., 2012).
Clustering and comparing multiple libraries whose size ranges from 250,000 to 850,000 molecules can be time consuming.
The genome of Db11 was shotgun sequenced to approximately 10-fold coverage from 90,142 end sequences from multiple shotgun libraries: pMAQ1Sac_ BstXI (with insert sizes of 5.5 6, 9 10, and 10 12 kb), pUC19 (with insert sizes of 1.4 2 and 2 2.8 kb), pOTWI2 (with insert sizes of 3 3.3 and 2 2.8 kb), and M13mp18 (with insert sizes of 1 1.4, 0.4 0.8, 1 1.4, and 0.5 1 kb).
As multiple Illumina libraries with different DNA fragment sizes were generated for this genome project (supplementary fig. S3, Supplementary Material online; libraries 24 27), we were able to evaluate how fragment size affects the quality of the assembly (table 1).
