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Sequence analysis of the multiple size cDNAs cloned from both seed coat and cotyledons revealed multiple transcripts derived from the wp allele containing the wild type gene (F3H1) exons (1, 2, 3) plus varying portions of exonic and intronic regions of the gene fragments captured by the Tgm-Express1 element that interrupts Intron 2 in the wp allele.
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For genes represented by multiple arrayed cDNAs, the average log2 ratio was used.
Median fluorescence ratios were calculated for genes represented by multiple arrayed cDNAs.
When multiple predicted cDNAs were assigned to the same gene lineage, the rpkm values were summed.
Multiple cDNAs encoding three distinct open reading frames were identified.
Due to redundancies in the assignment of multiple cDNAs to single genes, the 304 cDNAs corresponded to 268 different genes.
Multiple cDNAs confirm exonization of antisense sequences of FL elements (see [ 22]).
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