Sentence examples for multiple site incorporation from inspiring English sources

Indeed, the addition of 10 ng/μL o-tRNAopt DNA template to CFPS reactions increased pPaF incorporation into sfGFP in the presence of RF1, having a more pronounced effect on multiple site incorporation.

Of course, multiple site incorporations of tfmF and 19F-NMR will be required to reflect the uniform changes of the sample conditions, e.g. acidification, viscosity changes or protein degradation.

In addition, multiple site incorporations were much more efficient in the absence of RF1; we observed a 10-fold increase in the modified protein yield with two incorporation sites and a 12-fold increase with five incorporation sites.

We then used transzyme o-tRNAopt in single and multiple site pPaF incorporation in the rEc.E13 extracts with or without RF1 to measure if extra o-tRNAopt increases the modified sfGFP yield.

It is known that the presence of RF1 can result in the production of truncated proteins, particularly when multiple site-specific incorporations are desired, resulting in low yields of the target protein product.

Different from traditional multiple-site 19F incorporation through growing bacteria in media containing the 19F-aromatic residues (Lee et al., 2000), the 19F-containing unnatural amino acids could implement site-specific 19F labeling, resulting in the straight-forward resonance assignment (Hammill et al., 2007; Li et al., 2010).

To date, directed laboratory evolution of new biosynthesis ability using genetically encoded Uaas has not been feasible due to two demanding requirements: simultaneous Uaa incorporation at multiple sites and an autonomous host.

The new simulation involves the incorporation of monomer molecules which occupy multiple sites with their functional groups at the ends, initiator molecules which decay into two radicals per initiator, and the incorporation of a crankshaft-type motion for all of the species on a face-centered cubic lattice.

Indeed, major ESI-MS peaks of the purified protein suggest the incorporation of 9 into sfGFP at multiple sites (Supplementary Figure 3).

And you should stop reusing passwords on multiple sites.

Hierarchical solution multiple layers, which may consist of multiple sites.