Sentence examples for multiple significant hits from inspiring English sources
Genes having at least one significant hit were kept, and for genes with multiple significant hits, only the best one is reported.
Given TOCCATA's conservative grouping of SCOP and CATH hierarchies and the inherent similarity between several families, there are often multiple significant hits corresponding to various closely related profiles.
We initially explored marginal associations between genotypes and phenotypic and expression traits through whole-genome scans, and then, in genomic regions with multiple significant hits, we assessed gene-phenotype network reconstruction using causal structural learning algorithms.
Those putative precursor sequences that provided multiple significant hits against the salmon genome sequence (more than 5 hits with E-values <1× e-6) were removed (data not shown).
HHpred analysis initiated with the gp7 sequence returned multiple significant hits to Cas4 proteins (P = 96.3, E = 4.9e-03; Additional file 1: Figure S1B) and multiple sequence alignment is fully consistent with this result (Fig. 1b).
A multiple alignment of the significant hits revealed the presence of a conserved sequence motif (CPYCx(14,19 WxCxxCxRxF) containing four conserved cysteine residues.
When the BLAST of a given EST sequence search had highly significant hits on multiple RefSeq protein IDs, we found they were generally isoforms of the same gene.
Multiple BLAST searches using protein sequences of significant hits as the queries were then performed in several rice databases, including NCBI [ 69], TIGR [ 47], Rice Genome Database-japonica of the Rice Genome Research Program [ 83] and the International Rice Genome Sequencing Project (IRSGP) [ 84].
The HMM search of A. kona ORFs for domain similarities against multiple databases (Pfam, SCOP, and InterPro) also did not generate significant hits (E value threshold 1 e−).
Previously, we found the promoter regions of RASSF1 and WIF1 were methylated in over 60% of the NPC patients 12. Consistently, multiple CpG sites from promoter CGI of these two genes were identified as significant hits in the current study.
Multiple BLAST searches were then performed in several rice databases using rice protein sequences with significant hits as queries (see Methods for details).
