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To understand how ChpT interacts with multiple signaling partners, we solved the crystal structure of ChpT at 2.3 Å resolution.
Our unexpected result demonstrating the gain of function effects in the D36 mutant compared to the KO underscores the complexity of analyzing mouse mutants of the AKAP scaffolding proteins since they assemble with multiple signaling partners that may interact with each other as well as potential substrates.
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This may suggest there is a pressure towards the 'orphanisation' of some receiver-domain only genes, potentially because they have multiple signalling partners.
This multiple modular architecture enables regulatory selectivity and specificity in their mediation with diverse signaling partners that include all three components of the GPCR signaling pathway [41], [6].
We present a hybrid computational methodology to predict multiple energetically accessible conformations for G protein-coupled receptors (GPCRs) that might play a role in binding to ligands and different signaling partners.
These signals activate and suppress multiple signaling cascades regulating bone formation and resorption.
DNA damage activates multiple signaling cascades.
MEK Partner 1 (MP1 or MAPKSP1) is a scaffold protein that has been reported to function in multiple signaling pathways, including the ERK, PAK and mTORC pathways.
LncRNA H19, together with its partner miR-675, a miRNA embedded in its first exon, were proposed to regulate EMT through multiple signaling pathways, one of them being the PI3K AKT pathway [130].
SIRT1 also modulates multiple signaling factors.
RAGE ligation can activate multiple signaling pathways.
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