Sentence examples for multiple siRNAs targeting from inspiring English sources

Although verification of the siRNA target gene can be easily done by generating multiple siRNAs targeting other regions of the gene, this strategy becomes problematic if the phenotype caused by the siRNA is mediated strictly by an "off-target" effect.

siRNAs also possess additional features which make them highly attractive as pharmaceuticals, including 1) their high degree of specificity, 2) the ease with which multiple siRNAs targeting a single proteins can be designed, thereby reducing the likelihood of drug resistance, and 3) the ability to simultaneously target two or more gene products.

This was further validated using multiple siRNAs targeting HIF-1α (Fig  EV2C).

The same result was obtained using multiple siRNAs targeting different regions of EEF2K (Fig. 1B).

This was confirmed using multiple siRNAs targeting PHD2 (Fig EV3A, knock-down efficiencies shown in Fig EV3C).

Thirdly, loss of PHD2 prevented CAF-induced cancer cell invasion (Figs 5D and EV3D); this effect was confirmed using multiple siRNAs targeting PHD2 (Fig EV3E).

We present here a thorough characterisation of the efficacy of expressed lhRNAs designed to generate multiple siRNAs targeted to non-contiguous siRNA-susceptible regions of HIV-1.

Although multiple siRNA targets have been identified within the genomic coding region, targets within the untranslated regions (UTR), which encode cis-acting regulatory elements, remain relatively unknown.

These results indicate limitations in current theoretical prediction methods and suggest that a realistic RNAi effort still requires testing multiple siRNA targets to validate those that are effective.

The incentive of the study is based on the other hypothesis that encapsulation of multiple siRNA targets, e.g., TNF-α and CyD1, into NiMOS likely result in an even more potent effect on downregulation of pro-inflammatory cytokines, so that combined administration can multiply efficacy of the treatment.

Moreover, delivery of multiple different siRNAs targeting the HIV genome demonstrated further inhibition of virus production.