Sentence examples for multiple shRNAs targeting from inspiring English sources

We utilized multiple shRNAs targeting endogenous human DDX21 to effectively lower DDX21 protein expression.

To assess the long-term effects of DDX21 deficiency on proliferation and tumorigenesis, we utilized multiple shRNAs targeting endogenous human DDX21 to effectively knock down DDX21 protein expression in three highly proliferative breast cancer cell lines.

We note that other approaches have been used to leverage information from multiple shRNAs targeting the same gene, including redundant siRNA activity (RSA) analysis [ 23], RNAi gene enrichment ranking (RIGER) [ 24], and the Gene Activity Ranking Profile (GARP) score [ 17].

We identified 8753 shRNAs whose abundance was significantly different in control (DMSO) and RITA-treated cells based on following criteria: (i) P-value<0.1, (ii) FDR<0.3 and (iii) P-values of the weighted Z-scores, P(w Z)<0.1, which integrate the information from multiple shRNAs targeting a single gene, thus minimizing the impact of possible off-target effects.

To generate shRNA expression plasmids we first used the Whitehead Institute siRNA selection program to design shRNAs targeting multiple regions of Paupar and Pax6.

Our group, in collaboration with Dorre Grueneberg and Ed Harlow [ 41], conducted a pilot synthetic lethal screen with shRNAs targeting 88 different kinases and multiple isogenic cell line pairs that differed only with respect to VHL status.

The shRNAs targeting eIF4E2 and a control shRNA have been described previously (Tao and Gao, 2015).

In the novel shRNA library, there are about tens of different shRNAs targeting one gene, and thus significantly avoids the off-target effects.

Adenoviruses expressing shRNAs targeting mSirt3 were constructed.

This study used three P311 shRNAs, targeting different regions of the P311 transcript and one control shRNA contracted shRNA1, shRNA 2, shRNA 3 and control.

The remaining top hits were represented by shRNAs targeting potential novel regulators of GSK3 signaling and included two distinct shRNAs targeting dihydrofolate reductase (DHFR), two PSMD7 shRNAs, and single shRNAs targeting ELA2 and RNF10.