Sentence examples for multiple shRNA vectors from inspiring English sources

For awhile, scientists worked around the problem by simply using multiple shRNA vectors against each transcript they wanted to silence, in the hope that one or a combination of them would work well enough.

However, creating a suitable cloning plan becomes increasingly difficult in complex strategies requiring repeated insertions and or large recipient vectors such as in constructing multiple shRNA expression vectors for RNAi studies.

In this study, we tested a multiple shRNA expression strategy for different shRNAs using repeated promoters in a lentiviral vector.

To rule out the possibility of "off-target" effects of these vectors in causing drug resistance, two independent shRNA vectors for both ARID1A and SMARCE1 were tested in validation assays in multiple cell systems.

Hence, the construction of pENTR.hU6.hH1 would allow for easy transfer of subcloned shRNA cassettes to different gateway based expression vectors, shRNA targeting of multiple genes, and the increased knockdown of a single target using multiple shRNA sequences.

For HUVEC, shRNA vectors (SABiosciences) were nucleofected using Amaxa.

Briefly, the NKI shRNA library was designed to target 7914 human genes, using three shRNA vectors for every targeted gene, cumulating in a total of 23,742 shRNA vectors.

Briefly, HEK293 cells were transfected with shRNA vectors and packaging vectors (Sigma-Aldrich) using FuGENE6 (Roche).

This suggests them as promising candidates for multiple shRNA approaches.

Using the shRNA barcode technique we were able to quickly identify active shRNA vectors from a complex mixture.

To deliver shRNA expression construct into NB cell lines, commercially available lentiviral shRNA vectors were used.