Sentence examples for multiple sgRNAs targeting from inspiring English sources

Taking advantage of the multiplexable genome engineering feature of the CRISPR/Cas9 system, we sought to generate different kinds of immunodeficient mouse strains by embryo co-microinjection of Cas9 mRNA and multiple sgRNAs targeting mouse B2m, Il2rg, Prf1, Prkdc, and Rag1.

The authors used multiple sgRNAs targeting the same gene to improve gene disruption efficiency, which may potentially increase the off-target effects.

Significantly, when sgRNAs targeting multiple genes were simultaneously introduced into cells, robust multiplexed endogenous gene activation was achieved.

This is particularly useful when using multiple sgRNAs to target a single site to reduce the chance of choosing inefficient sgRNAs, since it is advisable to mutate each site in the repair template to prevent recutting (Kim et al. 2014).

When there are multiple sgRNAs for multiple genes, the amount per sgRNA was evenly divided among genes first, then among the sgRNAs targeting each gene.

Two different sgRNAs targeting the same gene are more synergistic than identical sgRNAs.

Cutting efficiency of Col9-a1 sgRNAs peaked at 0.8 and 1.1 for sgRNAs targeting the 1st and 29th exon, respectively.

Two sgRNAs targeting the exon of HTT5 were fused to pCAMBIA1300-pYAO Cas9 pCAMBIA1300-pYAO Cas9

sgRNAs targeting essential (sgEss) and non-essential (sgNon) genes were used as controls.

We synthesized four single-guide RNAs (sgRNAs) targeting the conserved regions of HBV.

The sgRNAs targeting exon3 of Rag2 were obtained from ToolGen, Inc. (Seoul, Republic of Korea).