A number of laboratories shared serum batches and used a common LIF source.
The extent of serum-mediated inhibition varied with the lot of serum used, with large margins of error seen among the serum batches tested.
Indeed, multiple studies in the past have shown that serum-free test media for ELISPOT assays that lead to low background, high detection rates and similar magnitude of antigen-specific T-cell responses as compared to media supplemented with pretested serum batches [ 14, 15].
The use of pre-tested serum batches, therefore, is a restricting factor in its own right.
However, serum batches are known to have unique compositions and immunological properties.
Although the variation between different HSA batches will probably be smaller compared to differences of serum batches, a pretesting of new HSA batches may become needed to control assay performance over time [ 22].
A single foetal calf serum batch was used for the entire period of the study.
You can make multiple batches for multiple pies at the same time.
Multiple batches rather than a single batch of the Immulite assay were used to test the samples.
For the leaf-tying caterpillars, intervals spanned multiple food batches, so leaf data were averaged (weighted by time fed on a batch) for each interval.
