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Multiple sequences were aligned using Clustal X [45].
The multiple sequences were aligned and compiled using the Sequencher software v4.7 (GeneCodes, Ann Arbor, Michigan).
If multiple sequences were found, we used the following criteria to select one: (1) greatest length (2) highest level of identity (3) oral cavity or airway provenance.
After multiple sequences were aligned, we implemented the PSO algorithm and the sliding window technique to examine the performance on biological test data, as described above.
Because multiple sequences were available from each species, we preferred the MCMC method that does not give any limitation to the number of sequences from a species.
Multiple sequences were aligned using CLUSTALW (1.8) [38] and adjusted using the alignment editor Se-Al (version 2.0; available from http://evolve.zoo.ox.ac.uk).ac.uk
Similar(10)
However, this method cannot be easily used when multiple sequences are included in the data.
After multiple sequences are aligned, numerous viable regions may be found and conserved.
These multiple sequences are termed miRNA variants, also named isomiRs [ 19– 23].
Multiple PX sequences are aligned using ClustalW (* for invariant, for conserved, for less conserved changes).
Deligotyping on multiple large sequences was performed using 10 loci.
More suggestions(15)
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