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Murasaki facilitates very efficient anchor generation across multiple sequences using arbitrary spaced seeds and runs on sequences several magnitudes larger than what BLASTZ can handle.
We edited each sequence electropherogram with EditView (ver. 1.01; Applied Biosystems) and concatenated the multiple sequences using AutoAssembler (ver. 2.1; Applied Biosystems).
Clustal alignment was performed on the multiple sequences using the Lasergene MegAlign program (DNASTAR, Inc .. (B) Phylogenetic relationship derived from the multiple sequence alignment.
Sequences were aligned using the Clustal V method of aligning multiple sequences using MegAlign (v. 3.11) available in the Lasergene sequence analysis package (v.1.02 for the Apple Computer DNASTAR Incc).
SiScan compares multiple sequences using a window-based approach by randomizing positions within a window and calculating z-scores [ 1]. 3Seq works on the principle of identifying signals that are less likely to occur under a clonal reproduction model [ 2].
Similar(55)
A common approach for the identification of anchors among multiple sequences, used by TBA [22], first detects anchors between every pair of sequences, and then progressively integrates pairwise anchors to form anchors across multiple sequences.
Transmembrane regions were predicted for multiple aligned sequences using PRALINE [ 58].
Module 3: Module 3 recovers annotation information from multiple homologous sequences using a novel multi-step approach.
All of the complete Monkey King sequences were used for multiple sequence alignment using ClustalW in MEGA 5 [ 46].
Amino acid and nucleotide sequences in fasta formats retrieved from the plant Chromatin Database (ChromDB) were also used for multiple sequence alignment using ClustalW2.
The protein sequences are used for the multiple sequence alignment using Clustalw2 and the phylogenetic tree is viewed by Njplot (Tamura et al. 2007).
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