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This enabled us to account for allelic variation, recent duplication events, and possible sequencing errors while also allowing reads to be mapped to a unique sequence rather than to multiple sequences, resulting in a more accurate estimation of abundance levels.
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In case of multiple sequences resulted from a single BLAST hit, the particular sequence that fulfils all the above seven criteria along with the maximum values of MFEI and R was chosen.
In case of multiple sequences resulted from a single BLAST hit, the one which fulfils all the seven criteria with the maximum MFEI and the maximum R was retained.
When the number of emergency tasks is large and the duration is longer, the addition of an emergency task may result in the deletion of tasks in multiple original task sequences, resulting in a decrease in profit.
In cases multiple identical sequences resulted from cloned PCR products of one accession, only one sequence was included in the data set.
Duplicate sequences resulting from multiple isoforms of the same protein were removed.
Multiple alignment of the consensus sequences resulting from each mapping provided a detailed view of SNPs and indel regions while avoiding false differences resulting from partial assemblies or from assembly and scaffolding errors.
We further reduced this number by excluding promoter with gaps greater than 200 bps and the blastclust program was used to confirm that this dataset did not contain multiple copies of the same DNA sequences resulting in 14,790 promoters.
First, sequences designated Fragment, Partial, or Pseudogene were removed from the multiple sequence alignment resulting in the data set called "100" (all 3,286 complete tubulin sequences, including archaeal and bacterial "tubulin" genes, accounting for 2,633 alignment positions).
However, in some samples this interpretation is difficult, i.e. when (almost) equally quantitative LH variants are present or when multiple sequencing primers result in the presentation of different dominant types.
For the trnH-psbA spacer there was also no statistical difference between using partial sequences versus complete sequences in the searches: partial sequences resulted in 28.6% multiple matches while complete sequences resulted in 26.5% multiple matches (Chi-square 0.04; df = 1; p = 0.8).
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