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A group of similar stems which is conserved across multiple sequences is referred to as a stem block.
A method and SPR Opt (SNP and PCR- RFLP Optimization) software to perform a comprehensive, whole-genome analysis to forensically discriminate multiple sequences is presented.
The alignment of multiple sequences is essential for many tasks in sequence analysis, e.g. protein structure and function prediction, similarity search and phylogenetic analysis (Pei, 2008).
Similar(57)
However, this method cannot be easily used when multiple sequences are included in the data.
Multiple sequences were aligned using Clustal X [45].
After multiple sequences are aligned, numerous viable regions may be found and conserved.
The multiple sequences were aligned and compiled using the Sequencher software v4.7 (GeneCodes, Ann Arbor, Michigan).
Multiple sequences were aligned using MUSCLE software [ 46].
Multiple sequences were aligned using the ClustalW program.
Sequences were assembled with BioEdit, and multiple sequences were aligned with Clustal W (http://www.ebi.ac.uk/).ac.uk/
For most taxa, multiple sequences were available, in total 94 ingroup sequences (Additional file 8).
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