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Multiple sequences comparisons were performed with PIPMAKER software [ 59].
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Multiple sequence comparisons of proteins of the LcrD/FlbF family allowed the design of primers that specifically amplify sequences coding for type III secretion components.
Multiple sequence comparisons were performed with CLUSTALW.
Pairwise as well as multiple sequence comparisons are computed.
Multiple sequence comparisons identified 15 consensus SNPs, eight of which caused amino acid changes.
Multiple sequence comparisons were carried out on the platform at [ 63, 64].
Multiple sequence comparisons to evaluate SNPs were done with Sequencher 4.1 (GeneCodes, Ann Arbor, USA).
Sequence comparisons were carried out using DOTTER [ 45] and multiple sequence comparisons were carried out using the CLUSTALX [ 46].
Multiple sequence comparisons were performed with MAFFT [ 46] http://align.bmr.kyushu-u.ac.jp/mafft/online/server/ and BLAST online applications.
Pair-wise sequence comparisons were carried out using DOTTER [ 27] and multiple sequence comparisons were carried out using the CLUSTALX [ 78].
Based on multiple sequence comparisons, two well-known conserved sequences LTNKYAEGYPGRRYYGG (61 77) and GGHLTHG (134 140) were also detected in AnSHMT [ 25].
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