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These were considered validated because both miRNAs had multiple sequenced variants that had predicted target sites encompassing the observed cleavage sites (Figure 1B; http://asrp.cgrb.oregonstate.edu/db/).edu/db/
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The clinical disease presumably results from the additive effects of multiple sequence variants from the mother and the foetus together with environmental factors.
A majority of the samples only contained one major HpSVd variant, but some contained multiple sequence variants.
Some analyses require condensing multiple sequence variants output by Trinity down to a single, predominant transcript.
Multiple sequence variants, i.e., isomirs, were detected for most of the miRNA transcripts.
For most of the signatures, multiple sequence variants were observed amongst the multiple clones of each product that were sequenced.
Respecting biological reality, however, would require miRBase to couple multiple sequence variants to a single miRNA record.
Thus, in the RACE reactions, we observed both multiple signatures mapping to the same gene, as well as multiple sequence variants of one gene carrying the same signature.
Given the presence of multiple sequence variants, the Bowtie2 parameter "-k 5000" was used in order to ensure that all mappings of each read are reported.
In addition, many of the signatures produced multiple sequence variants amongst the cloned RACE products, indicating the presence of yet more copies of the gene in question.
For virtually all miRNA transcripts detected, multiple sequence variants, i.e., isomirs, could be observed in the data, reaching up to 1002 unique sequence variants for the 'miRNA|ENSGALT00000042432|5p||sense' transcript in the HT and EM samples.
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