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†Consensus sequence derived from a multiple sequence protein alignment of 26 hemagglutinininin sequences from Nepal.
SCA measures the covariation between pairs of amino acids in large multiple sequence protein alignment of a given protein family.
For multiple sequence protein alignment users can choose one out of three popular multiple sequence aligners: MUSCLE [ 27], PRANK [ 28] and MAFFT [ 29].
To construct a reference-free multiple sequence protein alignment, the sequences were aligned using Muscle v3.7 software under default parameters (Edgar 2004).
Each residue in the GmRLK18-1 LRR protein was analyzed on the basis of (a) a sequence conservation score based on multiple sequence protein alignment and (b) the free energy difference between the naturally occurring residue and the potentially optimal residue at that position.
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Multiple sequence alignments, protein translation and phylogenetic analysis were performed on the basis of nucleotide sequences using the software MEGA4 [37] and BioEdit (http://www.mbio.ncsu.edu/BioEdit/bioedit.html).html
Multiple sequence alignments, protein translation, and phylogenetic analysis were performed with the DNAStar (DNAStar Inc., Madison, WI, USA) software package.
To explore the evolutionary relationship between Arabidopsis and rice NCX proteins, multiple sequence alignment of protein sequences was performed using ClustalW2 program and phylogenetic tree was generated using MEGA6 software (Additional file 1: Figure S1).
For patient PIC1362 1052829) multiple sequences per protein per timepoint were available, with small differences between each sequence.
After multiple sequence alignment the protein family is represented by a three dimensional primer data matrix X(0 N×M×L.
Multiple sequence alignments of protein sequences were carried out with ClustalX Version 1.83 [ 46].
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