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For each gene and taxon, multiple sequence fragments were obtained by sequencing with different primers.
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The time complexity of Algorithm 2 is O(| SW| × (O(PhylInfMeth) + n × m × (n + r))), where | SW| is the cardinality of the set of MSA (multiple sequence alignment) fragments examined by the sliding window procedure and O(PhylInfMeth) is the running time of the phylogeny inference method used to infer the tree T from the MSA fragment MSA f.
The bona fide sites can then be inferred by multiple mapped sequenced fragments.
The analysis using the ClustalW multiple sequence alignment showed that fragments recovered from the PLA2 sequence produced by the cDNA E6d and described by Tsai et al. [ 37] displayed 94% sequential homology (Table 2), except for a unique fragment that was not found (VTDCNPK).
If a match to PF02985 ("HEAT repeat") or PF00514 ("Armadillo/beta-catenin-like repeat") is found, the corresponding fragment in the multiple sequence alignment is considered a potential repeat fragment.
Most, if not all, sequenced metagenomes are complex mixtures of chromosomal and plasmid sequence fragments from multiple organisms, possibly from different kingdoms.
Low taxonomic diversity and/or highly expressed genes can lead to a high degree of data redundancy; that is multiple identical or nearly identical sequence fragments.
Multiple library preparation to produce sequence fragments of various sizes for each sample genome of interest would also increase the number and type of events that could be detected with this method.
Multiple sequence alignment of N-terminal fragments of FhaB homologs from the four loci of strain 2336 with those of Bordetella pertussis FHA and Proteus mirabilis HpmA showed that they contain several common features.
We used the "BLAST 2 SEQUENCES" tool to identify the correct position of sequence fragments in the multiple sequence alignment (MSA) for these incomplete sequences.
We aligned 16S fragments using the multiple sequence alignment program PRANK [ 41] with empirical base frequencies, the Tamura-Nei substitution model and default settings for gap penalties.
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