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Multiple sections from different mice were examined.
Ongoing studies are using up to ten multiple sections from the tumour material.
Multiple sections from at least eight different flies of each genotype and age were examined for each experiment.
Analysis of multiple sections from wild-type pons revealed a complete absence of these larger diameter aggregates.
We do show a representative image based on inspection of multiple sections from n = 3 of the 6 allele mutant mice.
MetaMorph software was used to analyze total tumor burden and osteoclast number in multiple sections from the femora and tibiae of the hind limbs.
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For all experiments, multiple sections obtained from each treatment group and the average number of positive cells/area was calculated from 5 fields of each section.
However, as LGE-MRI requires expensive specialist equipment, assessment of experimental infarct injury more typically relies on 2D histological assessment in a single section or multiple sections collected from the infarct area.
Samples were taken from areas with no haemorrhage or necrosis, and multiple sections were submitted from the same tumour to compensate for tumour heterogeneity.
Multiple sections (n ≥20) from multiple tissue blocks (n ≥2) were examined to obtain representative views.
Multiple sections were cut from serial levels of the lung to ensure that the trachea and the complete bronchial stem including its branching points were available for systematic investigation.
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