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Routine histological and immunohistochemical examinations were made on multiple sections, at different levels throughout the paraffin-embedded tissue, to ensure assessment of any heterogeneity.
Routine histological and IHC examinations were made on multiple sections, at different levels throughout the paraffin-embedded tissue, to ensure assessment of any heterogeneity.
For this purpose, the tissue block was completely trimmed up into serial sections of 5 μm, and multiple sections at 50-μm intervals were immunostained for PrPSc.
With the lack of an obvious difference in amyloid plaque burden in APPswe/PS1dE9 mice lacking LRP1 expression in the hippocampus, we sought to carefully quantify plaque numbers in the hippocampus of multiple sections at multiple levels.
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Our sources in the rafters told us that multiple sections, particularly at stage left, were rocking.
Multiple sections taken at several levels for each case were reviewed following routine staining.
Multiple sections from at least eight different flies of each genotype and age were examined for each experiment.
To represent data from the entire hippocampus multiple sections were imaged at different levels through the structure using an EVOS FL Auto microscope (Life Technologies).
More than three fields of microscopic view in each section, and multiple sections (more than three) from at least six different experimental animals in each experimental group were used.
Finally, we note that the kidney and lung datasets analyzed here contain multiple sections of a solid tumor obtained at a single time point, whereas the CLL datasets contain samples obtained at different times.
Because professors at West Point teach multiple sections of the same class in a given semester, the researchers assigned each professor to more than one treatment condition.
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