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Unless multiple sections are collected throughout the length of the infarct, these histological approaches give at best an estimate of infarct injury rather than an actual measurement of infarct volume.
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The group, which he sensed was large and had multiple sections, was led by an Arab in his 40s who had fought in Afghanistan against the Soviet occupation.
The biopsy (0.3 × 0.2 × 0.1 cm) was fixed in 4% formaldehyde and processed entirely in paraffin, and multiple sections were cut and stained with hematoxylin and eosin as well as Brown and Brenn tissue gram stain, Ziehl-Neelsen stain, and Fite's acid-fast stain for mycobacteria, and Grocott's modified Gomori's methenamine silver stain for fungal elements.
Material from multiple sections was pooled, and all LCM samples were collected within 2 hr of sectioning.
Multiple sections were prepared and stained with periodic acid silver methenamine (PASM) and periodic acid-Schiff's reagent (PAS).
The effect of treatment on cell proliferation was investigated using a generalised linear mixed effects model where multiple sections were nested within individuals in order to take account of the nested nature of the data.
The effect of treatment on the cell proliferation was statistically analysed using a generalised linear mixed effects model where multiple sections were nested within individuals in order to take account of the nested nature of the data (R statistical package).
Additional multiple sections were cut for immunohistochemical analysis.
Metastases spanning multiple sections were only counted in the first section where they were identified.
Multiple sections were examined histologically for the presence of polymorphonuclear leucocytes as evidence of acute inflammation.
Multiple sections were stained with hematoxylin and eosin for morphological analysis.
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