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Due to insufficient clinical data one additional multiple sclerosis case could only be classified as progressive multiple sclerosis.
The only positive signal for EBER was seen in a few cells within a single lymphocyte-rich region in a single block of a single multiple sclerosis case.
Comparing this single multiple sclerosis case with all other cases included in our gene expression study allowed us to specifically examine the inflammatory component of this active cortical lesion.
We then established the age and regional origin distribution for a typical multiple sclerosis case collection and determined the false-positive rate expected when comparing such a collection with birth rates estimated by averaging population-specific national statistics.
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In an extension analysis of the 835 case-father pairs plus the original 159 multiple sclerosis cases and 671 controls, we found a trend towards an over-representation of super-haplogroup U (Table 3).
In our analysis of the five most common haplogroups, the finding of a trend towards an over-representation of super-haplogroup U (which encompasses haplogroups U and K) and an under-representation of the J/T haplogroup in cases, is in line with a study of multiple sclerosis cases in the Basque people [18].
In 17/67 multiple sclerosis cases large hemispheric or double hemispheric sections were additionally available.
The ages of onset in multiple sclerosis cases span more than 7 decades.
For multiple sclerosis cases, we micro-dissected cortical areas with subpial demyelination of at least the outer four cortical layers.
Further, 139 areas of NAWM, 121 areas of meninges and 120 areas of cortex were analysed in multiple sclerosis cases.
The cause of death for all multiple sclerosis cases and controls (Table 1) was either cardiorespiratory failure/arrest or pneumonia.
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