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Finally, we use multiple sampling across the same normal liver to ascertain whether DNA methylation patterns are consistent/stable across the organ and what changes, if any, might be associated with age and gender.
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SneakPeek is a quality metrics management system for reviewing the sequencing quality of multiple samples across different flow cells.
This indicated that most peptides were being identified in multiple samples across time points rather than an overrepresentation of a few technical replicate samples.
Unfortunately, we were unable to ascertain glucagon levels at this age as multiple samples across both wild-type and Smn +/− mice were below detectable levels (data not shown).
Temporal estimators use allele frequency data from multiple samples, across generations, to calculate N e (Nei and Tajima 1981; Pollack 1983; Waples 1989; Wang 2001).
The single colour workflow feature of GeneSpring GX was used in order to split the two channel array into 2 single colour experiments to enable the analysis of multiple samples across different arrays.
With regard to the influence of genetic and environmental factors across the day, this study—as have other twin studies with multiple samples across the day found stronger genetic influences in the morning and predominantly non-shared environmental influences later in the day (Bartels et al. 2003a, b; Kupper et al. 2005).
Consequently, more measures will be required from short-term measurement methods to capture variation within a day, but multiple samples across many days offers additional information about the robustness of the emissions phenotype that is not normally obtained by RC studies made only over 1 to 3 days.
We also report on the reproducibility of associations across alternate phenotyping and genotyping platforms, as well as across multiple sampling environments and varying plant ages.
These two components of floodplain surface complexity were measured across multiple sampling scales from LiDAR-derived DEMs.
Additionally, linear mixed-effects models were also used to assess the effect of 'Location', 'Season', and an interactive effect of 'Location' and 'Season' on soil moisture and soil nutrients across multiple sampling years.
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