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In both assays, multiple sample dilutions were compared to standard curves of wildtype DNase I to determine concentrations.
For ELISA measurements, multiple sample dilutions are required to cover this range, making reliable measurements of serum/plasma and synovial fluid (SF) IL-20 challenging.
PHM and PAL activity were assayed in duplicate using [I]-labeled substrate (Acetyl-Tyr-Val-Gly), 0.5 μM unlabeled substrate and multiple sample dilutions as described [ 51].
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Higher sample dilutions were made by dilution of diluted sample (1/20) in diluted plasma (1/20).
Adaptable dilution protocols allow the analysis of sample dilutions ranging from 1 2 to 1 2 × 107.
Standard curve and test sample dilutions were prepared in triplicate.
All sample dilutions were spotted in duplicate.
Serum sample dilutions of 1 8 to 1 1,024 were assayed, and each dilution was tested in duplicate.
Serum sample dilutions were prepared in EMEM containing 2% FBS.
Serum samples were diluted in sample dilution buffer (1 200 dilutions for bovine samples and 1 2,000 dilutions for sheep/goat samples).
Samples were diluted 1 5 using the sample dilution buffer.
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