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All results are averaged over multiple runs on multiple random topologies.
After multiple runs on each K assumed, the value that generated the highest posterior probability was used as the number of possible populations.
Unfortunately, the current implementation of LSA requires multiple runs on permuted data and a Monte Carlo statistical method known as a permutation test to evaluate a null distribution and obtain a p-value determining significance.
From 24 isolates, which were cultivated on HEp-2 cells and sequenced in multiple runs on an Illumina GA II sequencer, 19 yielded a sufficient amount of reads for genome assembly (Table 1).
Mass measurement errors are estimated using an annotated library of reference metabolites which are obtained from multiple runs on the same MS instrument, containing information on peaks of related features such as isotopes and adducts.
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In some cases, de novo gene predictions from genscan or evidence-based prediction with N-scan (UCSC Genome Browser, accessed 17 April 2015) did identify overlapping open reading frames, however, these annotations were typically of poor quality (data not shown) with multiple run-on annotations linking two or more MHC class I gene features.
The consensus cluster algorithm is based on an averaging procedure conducted on multiple runs of K-means clustering (see " Methods").
Multiple runs based on the same priors were then combined in L-mode and log-likelihood ratio tests were performed to test for isolation.
Trees inferred from duplicate BEST runs were identical in topology with no nodes differing by Pp > 0.05, indicating that multiple runs converged on nearly the same posterior tree space.
We evaluated multiple runs in BEAST which converged on the same estimates.
Multiple runs were performed on the same day, with sexes tested on separate days, and the 200 most resistant and most sensitive males and females were collected.
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