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Phylogeny was inferred using the Markov chain Monte Carlo package Mr Bayes (http://mrbayes.csit.fsu.edu/) with the HKY model that is parametrised by nucleotide frequencies, branch lengths and the transition-transversion ratio Κ. Convergence was confirmed using a multiple run methodology [19].
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Application of a multi-threshold run methodology can effectively solve the main problems of drought identification.
Multiple runs were conducted.
Typically, past history would remain in place across multiple runs.
All results are averaged over multiple runs on multiple random topologies.
Multiple runs (generally 3) were run to avoid spurious results.
Convergence and stationarity were compared between multiple runs.
Multiple runs of the program consistently provided the same results.
Data are collected from multiple runs with duplicate templates.
We conducted multiple runs for each parameter set to ensure convergence between runs.
The multiple model methodology may be useful here as well.
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