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As part of the studies conducted by the OECD validation initiative, it has been established that the phytoestrogen contents of the multiple rodent diets employed by the participant laboratories had no important effect on the sensitivity of the uterotrophic assay (Owens et al. 2003).
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Hantavirus enzootics are maintained in multiple rodent species [5] [8].
These levels are typical of those previously found in rodent diets [16,44].
The removal of soybean meal from all rodent diets is another potential option.
However, the primary known source of active estrogenic chemicals present in most rodent diets is isoflavones.
Researchers say it should be no surprise that a virus capable of infecting multiple rodents in Africa could find a ready host in a rodent here.
Rodent diet and water were provided ad libitum.
The standard rodent diet used by the test laboratory contains approximately 33% maize grain.
Animals had unrestricted access to food (Teklad Global Rodent Diet, Harlan, Madison, WI, USA) and water.
The folate-free diet was replaced by a standard rodent diet (Kliba Nafag, Kaiseraugst, Switzerland) 3 weeks after application of the radiofolates.
Female CD-1 nude mice (Charles River, Sulzfeld, Germany) were fed with a folate-deficient rodent diet (Harlan Laboratories, Indianapolis, IN, USA).
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