Both parties expect the multiple restrictions on the conduct of the war to fall short of the 60 votes required to break a filibuster.
Even multiple restrictions, where several of the restrictions that remove solutions from the solution space were imposed simultaneously, realised 89% of the additional gain.
We also simulated a breeding scheme, multiple restrictions, in which several of the restrictions that remove solutions from the solution space were imposed simultaneously.
The exceptions were pre- selection of sires 0.5 and 1.0% and multiple restrictions.
Lifting multiple restrictions could transform the economy and substantially improve prospects for sustained growth".
There is no evidence of germline transmission, no evidence of functional contribution, and uncertainty about the contribution of multiple restriction sites to the generation of the reported fragments.
DADA is based upon multiple restriction fragment length analysis of pools of clones from cDNA libraries and the identification of gene-specific restriction fingerprints in the resulting complex fragment mixtures.
The array of multiple restriction endonuclease sites provides versatile possibilities for the choice of the genomic restriction fragments utilized and for the final linearization procedure prior to the delivery into ES cells.
Lebedenko et al reported an in vitro exon ligation method, but it involved complicated steps including two-step PCR to amplify the exons, cloning of all exons separately, introduction of specific restriction enzymes and ligation of multiple restriction fragments[25].
The use of multiple restriction enzymes for TMDH has theoretical advantages, but increases the expense of the method since it involves an additional set of microarrays.
