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Another evident characteristic of the S. macedonicus genome was the presence of multiple restriction modification (RM) systems among the singleton genes (Additional file 9: Figure S4).
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Type1 restriction modification system are intriguing multifunctional multisubunit molecular motors that can catalyze both restriction and modification activity.
In experiment series 1, the products from R+ cultures were examined for restriction modification phenotype in a phage restriction assay.
Type I restriction modification systems consist of the R, M, and specificity (S) subunits (Fig. 1B) [1B].
Restriction modification systems are a way for bacteria to control the uptake of foreign DNA.
A novel restriction modification system was annotated on the genome of DPC6026.
Multiple restriction fragments were observed after hybridization of BvMSat08.
The plasmid constructs were verified by multiple restriction analysis.
The obtained plasmid constructs were verified by multiple restriction analysis.
Multiple restriction enzyme usage is recommended for better resolution.
Coverage can be improved by using multiple restriction endonucleases [164].
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