Sentence examples for multiple restriction enzymes from inspiring English sources

This means that to survey widely distributed mutations multiple restriction enzymes would have to be used in a manner that does not interfere with subsequent PCR amplification of the resulting fragments.

The identities of the amplified fragments were verified using digestion with multiple restriction enzymes as well as sequencing.

In other classification systems, numerical codes for multiple restriction enzymes are used to denominate the genome type.

User-defined RRBS restriction enzymes (multiple restriction enzymes are supported) are used to digest the references into 40 220 bp segments (subsequently called 'target regions').

Of course, selection of one or multiple restriction enzymes for reduced representation library also relies on genome features in a given organism and applications.

To introduce multiple DNA fragments into a vector we need to use multiple restriction enzymes but sometimes enough restriction enzymes sites may not be available.

Multiple restriction enzyme usage is recommended for better resolution.

When restriction enzymes overlap in one or more base pairs, this motif may contain multiple restriction enzyme recognition sites, producing cDNA fragments shorter than the 50 bp required for visualization.

Here, we report the addition of a single-step multiple restriction enzyme digestion (MRED) designed to differentially digest polymerase chain reaction products amplified from unconverted DNA while leaving those of converted DNA intact.

We, therefore, aimed to circumvent these issues altogether by depleting the PCR populations of products amplified from partially converted or unconverted DNA using a multiple restriction enzyme digestion (MRED) approach.

After digestion with multiple 4-cutter restriction enzymes, enrichment for SSRs containing fragments was obtained through biotinylated TCn, TGn, and GATAn simple sequence motifs.