Sentence examples for multiple restriction endonucleases from inspiring English sources

Manipulation of the large genomic DNA is tedious and problematic due to size capacity of cloning vectors and multiple restriction endonucleases which make it difficult to find appropriate enzymes for subcloning.

Coverage can be improved by using multiple restriction endonucleases [164].

The SNaPshot HICF fingerprinting technology is based on restriction digestion of the DNA of each BAC clone by multiple restriction endonucleases and sizing a portion of the fragments with capillary electrophoresis.

The array of multiple restriction endonuclease sites provides versatile possibilities for the choice of the genomic restriction fragments utilized and for the final linearization procedure prior to the delivery into ES cells.

In order to obtain an artificial promoter library suitable for cloning, non-degenerated flanks that carry multiple recognition sites for restriction endonucleases (Multiple Cloning Site MCS) were added to both ends of the single-stranded DNA sequence.

Type II restriction endonucleases always cleave at or near their recognition sites.

Restriction endonucleases are a special class that recognize and cleave specific sequences in DNA.

Fragments of DNA produced by restriction endonucleases can be moved from one organism to another.

restriction endonucleases.

Restriction endonucleases were obtained from Fermentas (Waltham, USA).

Restriction endonucleases were purchased from New England Biolabs and ligases were purchased from Invitrogen (Carlsband, CA).