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Calcium-activated Cn dephosphorylates multiple residues within the regulatory domain of NFAT, triggering joint nuclear translocation.
In this study, we identify specific regions and multiple residues within Hhat that regulate protein stability and/or catalysis.
This in turn results in the autophosphorylation of multiple residues within the intracellular region [4], a mechanism common to various tyrosine kinases, leading to downstream signaling and changes to cell phenotype.
Finally, and quite significantly, Ii1's hydroxamate moiety, amide backbone, and P2' residue all make substantial interactions not only with residues in the N-terminal portions of IDE involved in zinc-binding and catalysis, but also with multiple residues within distal C-terminal portions of the protease (Fig. 4B; Fig. S4).
Experiments in which either single or multiple residues within the L2 aromatic region were substituted resulted in a several fold decrease in Hb binding, loss of Hb binding to S. aureus cells, or a significant decrease in the rate of in vitro heme transfer from metHb to IsdB.
The oxidative modification of proteins by ROS has been classified in two groups; (a) Global oxidative modifications, which include oxidation of multiple residues within protein to form several products; (b) Specific oxidative modifications which are very specific in both the residue oxidized and the product generated, e.g. oxidation of tyrosine residue to give dityrosine [ 14].
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Furthermore, the picture portrayed by our results of sparse networks of multiple residues driving specificity together would fit within the scope of more recent theories on protein function, namely, the so-called protein sector model.
Engineering histones with defined PTMs on one residue or on multiple residues is crucial for understanding their function within nucleosomes and chromatin.
The issue of multiple residues should also be addressed.
Phospho-peptide mapping revealed that XopE2 is phosphorylated at multiple residues in planta and residues T66, T131 and S334 are required for maximal binding to TFT10.
Brackets indicate ligands composed of multiple residues or fragments.
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