The αB crystallin mutant αAβ8, which contains multiple mutations at residues corresponding to the 131LTITSSLS138 sequence increased microtubule assembly, completely inhibited microtubule disassembly, and decreased tubulin aggregation.
The aligned tRNAGln sequences are given in Additional file 7. The N-terminal catalytic domain and the C-terminal anticodon-binding domains of GluRS were defined from multiple aligned GluRS sequences by annotating residues corresponding to 1-322 and 323-468 of T. thermophilus GluRS as the N- and C-terminal domains, respectively [ 10].
Multiple sequence alignment of sequences of ∼620 residues, corresponding to the last three EGF domains to C-terminus of these proteins (or, to include Nv22035 in the analysis, only the residues corresponding to the type 3 repeats and the L-lectin domain), was made in MUSCLE 3.7.
A multiple alignment using ClustalX showed that the conserved cysteine residues, including the residues corresponding to AtNPR1 Cys82 and Cys216, which are required for oligomerization, were also present in PhaNPR1 (Figure 1B).
We extracted the 22 residues corresponding to the putative odorant binding site from each OR sequence in the multiple alignment [25].
The issue of multiple residues should also be addressed.
c) The amino acid residues corresponding to the codons between 258 and 263 of the mouse FS, were also extracted, and the residues that are different from the consensus FYYGGA sequence are indicated in bold.
Phospho-peptide mapping revealed that XopE2 is phosphorylated at multiple residues in planta and residues T66, T131 and S334 are required for maximal binding to TFT10.
Brackets indicate ligands composed of multiple residues or fragments.
Fitting problems can best be diagnosed by validation that spans multiple residues; CaBLAM is such a multi-residue tool, and its effectiveness is demonstrated.
