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Most genera are represented by a single species but multiple representatives are included for highly diversified or potentially paraphyletic genera.
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Cluster representatives were chosen based on phrap quality scores to use the highest quality sequences for further analysis; for large clusters, multiple representatives were chosen.
Multiple representatives were selected for those families where a single BRS gives low coverage.
Multiple representatives were included for all large genera and species groups (Additional file 1).
There are multiple HDIs in clinical development and key representatives are shown in Figure 1C.
In tumours with heterogeneous ERCC1 staining, multiple representative areas were scored; staining intensity was assigned as a weighted sum rounded to the nearest quartile.
Multiple representative colonies were inoculated into YPD broth and were starter cultures for the stress resistance assays.
To detect luminal epithelial cells undergoing apoptosis in glands from wild-type or Brk mice, IHC was performed with antibodies directed against cleaved caspase 3. Multiple representative images were scored for positive cells, with visible dead cells already shed into the lumen also being counted as positive.
During the entire process, access to multiple analysts and industry representatives was purposefully employed in order to increase the validity of the results.
Multiple representative embryos were imaged on a Zeiss LSM 510 Meta with an LD C-Apochromat 40x/1.1 W Corr objective.
The multiple sequence alignment of these representatives was made by PROMALS that uses information of predicted secondary structures and database homologs [ 70].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com